🇨🇿 Státní zdravotní ústav PL
Microbiology
Státní zdravotní ústav is a medical laboratory accredited by ČIA to ISO 15189 under number 8002 (Praha, Czech Republic). Its accreditation scope covers 79 examinations. Examined materials include: Bacterial culture, Clinical material, Serum, plasma, Body fluids. Most frequently used examination principles: Immunoassay with photometric detection, Real-time PCR, Agglutination.
At a glance
- Accreditation number: ČIA 8002
- Examinations in scope: 79
- Types of examined material: 11
- Examination principles used: 31
- City: Praha, Czech Republic
- Valid until: 11-12-2029
- Accreditation number
- 8002
- Accreditation body
- ČIA
- Address
- Šrobárova 49/48, 100 00 Praha 10 - Vinohrady
- City
- Praha, Czech Republic
- Division
- Laboratories of the Centre for Epidemiology and Microbiology (LCEM)
- Valid until
- 11-12-2029
Materials examined by this laboratory (11)
- Bacterial culture — 21 examinations
- Clinical material — 21 examinations
- Serum, plasma — 13 examinations
- Body fluids — 9 examinations
- Mycobacterial culture — 7 examinations
- Plasma — 2 examinations
- Serum, plasma, cerebrospinal fluid, synovial fluid — 2 examinations
- Blood, cerebrospinal fluid, lesion swab, urine, bronchoalveolar lavage aspirate, conjunctival swab, leukocytes, autopsy material, infected tissue cultures, ocular fluid, punctates, bone marrow, amniotic fluid — 1 examination
- Cerebrospinal fluid, synovial fluid, tissue biopsies, urine, plasma, anticoagulated blood — 1 examination
- Serum, plasma, cerebrospinal fluid — 1 examination
- Swabs, vesicular fluid, urine, bronchoalveolar lavage — 1 examination
Examination principles (31)
- Immunoassay with photometric detection — 12 examinations
- Real-time PCR — 11 examinations
- Agglutination — 7 examinations
- Phenotyping — 7 examinations
- Immunoblotting — 5 examinations
- Indirect immunofluorescence — 4 examinations
- Direct sequencing (Sanger) — 3 examinations
- Cultivation on tissue cultures — 2 examinations
- DNA Strip technology (line probe assay) — 2 examinations
- End-point PCR with gel electrophoretic detection — 2 examinations
- Immunoassay with fluorimetric detection (automated) — 2 examinations
- Immunoassay with luminometric detection (automated) — 2 examinations
- Microdilution method; E-test — 2 examinations
- Aerobic culture; microscopy — 1 examination
- Complement fixation reaction — 1 examination
- and 16 more
Laboratory sections (21)
- National Reference Laboratory for HIV/AIDS (NRL/HIV/AIDS) — 8 examinations
- National Reference Laboratory for Mycobacteria (NRL/M) — 8 examinations
- National Reference Laboratory for Syphilis Diagnostics (NRL/SYF) — 6 examinations
- National Reference Laboratory for Viral Hepatitis (NRL/VH) — 6 examinations
- National Reference Laboratory for Antibiotics (NRL/ATB) — 4 examinations
- National Reference Laboratory for Chlamydiae (NRL/CHLA) — 4 examinations
- National Reference Laboratory for Herpesviruses (NRL/HV) — 4 examinations
- National Reference Laboratory for Lyme Borreliosis (NRL/LB) — 4 examinations
- National Reference Laboratory for Meningococcal Infections (NRL/MEN) — 4 examinations
- National Reference Laboratory for Pertussis and Diphtheria (NRL/DIPE) — 4 examinations
- National Reference Laboratory for Streptococcal Infections (NRL/STR) — 4 examinations
- National Reference Laboratory for Toxoplasmosis (NRL/TOX) — 4 examinations
- National Reference Laboratory for E. coli and Shigellae (NRL/ECS) — 3 examinations
- National Reference Laboratory for Enteroviruses (NRL/ENT) — 3 examinations
- National Reference Laboratory for Staphylococci (NRL/ST) — 3 examinations
- and 6 more
Laboratory locations
- Státní zdravotní ústav (main site) — Šrobárova 49/48, 100 00 Praha 10 - Vinohrady
- National Reference Laboratory for HIV/AIDS (NRL/HIV/AIDS) — Šrobárova 49/48, 100 00 Praha 10
- National Reference Laboratory for Antibiotics (NRL/ATB) — Šrobárova 49/48, 100 00 Praha 10
- National Reference Laboratory for Mycobacteria (NRL/M) — Šrobárova 49/48, 100 00 Praha 10
- National Reference Laboratory for Lyme Borreliosis (NRL/LB) — Šrobárova 49/48, 100 00 Praha 10
- National Reference Laboratory for Viral Hepatitis (NRL/VH) — Šrobárova 49/48, 100 00 Praha 10
- National Reference Laboratory for Meningococcal Infections (NRL/MEN) — Šrobárova 49/48, 100 00 Praha 10
- National Reference Laboratory for Haemophilus Infections (NRL/HEM) — Šrobárova 49/48, 100 00 Praha 10
- National Reference Laboratory for Streptococcal Infections (NRL/STR) — Šrobárova 49/48, 100 00 Praha 10
- National Reference Laboratory for Influenza and Non-Influenza Respiratory Viral Diseases (NRL/CH a NCH) — Šrobárova 49/48, 100 00 Praha 10
- National Reference Laboratory for Syphilis Diagnostics (NRL/SYF) — Šrobárova 49/48, 100 00 Praha 10
- National Reference Laboratory for Detection of Infectious Agents by Electron Microscopy (NRL/ELM) — Šrobárova 49/48, 100 00 Praha 10
- National Reference Laboratory for Enteroviruses (NRL/ENT) — Šrobárova 49/48, 100 00 Praha 10
- National Reference Laboratory for Herpesviruses (NRL/HV) — Šrobárova 49/48, 100 00 Praha 10
- National Reference Laboratory for Rubella, Measles, Mumps and Parvovirus B19 (NRL/ZSP) — Šrobárova 49/48, 100 00 Praha 10
- National Reference Laboratory for Chlamydiae (NRL/CHLA) — Šrobárova 49/48, 100 00 Praha 10
- National Reference Laboratory for Leptospires (NRL/LEPT) — Šrobárova 49/48, 100 00 Praha 10
- National Reference Laboratory for Toxoplasmosis (NRL/TOX) — Šrobárova 49/48, 100 00 Praha 10
- National Reference Laboratory for Staphylococci (NRL/ST) — Šrobárova 49/48, 100 00 Praha 10
- National Reference Laboratory for Salmonellae (NRL/SA) — Šrobárova 49/48, 100 00 Praha 10
- National Reference Laboratory for E. coli and Shigellae (NRL/ECS) — Šrobárova 49/48, 100 00 Praha 10
- National Reference Laboratory for Pertussis and Diphtheria (NRL/DIPE) — Šrobárova 49/48, 100 00 Praha 10
Certificate information
- Certificate date
- 11.12.2024
Accreditation scope — medical laboratory examinations (79)
| No. | Examined material | Analyte / parameter / diagnosis | Examination principle | Examination procedure / equipment | |
|---|---|---|---|---|---|
| National Reference Laboratory for HIV/AIDS (NRL/HIV/AIDS) | |||||
| 1 | Serum, plasma | HIV markers 802 – Medical Microbiology |
Immunoassay with photometric detection | SOP-NRL/HIV/AIDS-03, 1. 8. 2024; CIE10.NRL/HIV/AIDS, 1. 8. 2023 | |
| 2 | Serum, plasma | HIV markers 802 – Medical Microbiology |
Immunoassay with fluorimetric detection (automated) | SOP-NRL/HIV/AIDS-05, 1. 8. 2024; CIE10.NRL/HIV/AIDS, 1. 8. 2023; miniVIDAS | |
| 3 | Serum, plasma | HIV-1 p24 antigen 802 – Medical Microbiology |
Immunoassay with fluorimetric detection (automated) | SOP-NRL/HIV/AIDS-06, 1. 8. 2024; CIE10.NRL/HIV/AIDS, 1. 8. 2023; miniVIDAS | |
| 4 | Serum, plasma | HIV markers 802 – Medical Microbiology |
Immunoassay with chemiluminescent detection (automated) | SOP-NRL/HIV/AIDS-21, 1. 8. 2024; CIE10.NRL/HIV/AIDS, 1. 8. 2023; Architect System i1000 | |
| 5 | Serum, plasma | Antibodies against an infectious agent 802 – Medical Microbiology |
Immunoblotting (automated) | SOP-NRL/HIV/AIDS-07, 1. 8. 2024; CIE10.NRL/HIV/AIDS, 1. 8. 2023; Dynablot 44 | |
| 6 | Plasma | Detection of nucleic acids of infectious agents 802 – Medical Microbiology |
Quantitative real-time PCR | SOP-NRL/HIV/AIDS-19, 1. 8. 2024; CIE10.NRL/HIV/AIDS, 1. 8. 2023; cobas 4800 | |
| 7 | Plasma | Virus genotype 802 – Medical Microbiology |
Direct sequencing (Sanger) | SOP-NRL/HIV/AIDS-15, 1. 8. 2024; CIE10.NRL/HIV/AIDS, 1. 8. 2023 Applied Biosystems 3500 | |
| 8 | Serum, plasma | Antibodies against an infectious agent 802 – Medical Microbiology |
Immunochromatography | SOP-NRL/HIV/AIDS-08, 1. 8. 2024; CIE10.NRL/HIV/AIDS, 1. 8. 2023 | |
| National Reference Laboratory for Antibiotics (NRL/ATB) | |||||
| 9 | Bacterial culture | Quantitative susceptibility testing of microorganisms 802 – Medical Microbiology |
Microdilution method; E-test | Published procedure | |
| 10 | Bacterial culture | Qualitative susceptibility testing of microorganisms 802 – Medical Microbiology |
Disc diffusion test | Commercial procedure; published procedure | |
| 11 | Bacterial culture | Examination of mutations in susceptibility/resistance genes 802 – Medical Microbiology |
Real-time PCR | Published procedure | |
| 12 | Bacterial culture | Examination of mutations in susceptibility/resistance genes 802 – Medical Microbiology |
End-point PCR with gel electrophoretic detection | Published procedure | |
| National Reference Laboratory for Mycobacteria (NRL/M) | |||||
| 13 | Clinical material | Detection of mycobacteria 802 – Medical Microbiology |
Aerobic culture; microscopy | Published procedure | |
| 14 | Mycobacterial culture | Quantitative susceptibility testing of mycobacteria 802 – Medical Microbiology |
Microdilution method; E-test | Published procedure; commercial procedure | |
| 15 | Mycobacterial culture | Identification of mycobacteria 802 – Medical Microbiology |
DNA Strip technology (line probe assay) | Commercial procedure | |
| 16 | Mycobacterial culture | Identification of mycobacteria 802 – Medical Microbiology |
Real-time PCR | Commercial procedure | |
| 17 | Mycobacterial culture | Examination of mutations in susceptibility/resistance genes 802 – Medical Microbiology |
DNA Strip technology (line probe assay) | Commercial procedure | |
| 18 | Mycobacterial culture | Examination of mutations in susceptibility/resistance genes 802 – Medical Microbiology |
Real-time PCR | Commercial procedure | |
| 19 | Mycobacterial culture | Qualitative susceptibility testing of mycobacteria 802 – Medical Microbiology |
Proportion method | Published procedure | |
| 20 | Mycobacterial culture | Qualitative susceptibility testing of Mycobacterium tuberculosis complex 802 – Medical Microbiology |
Metabolic method | Commercial procedure | |
| National Reference Laboratory for Lyme Borreliosis (NRL/LB) | |||||
| 21 | Serum, plasma, cerebrospinal fluid, synovial fluid | Antibodies against an infectious agent 802 – Medical Microbiology |
Immunoassay with photometric detection | Commercial procedure | |
| 22 | Serum, plasma, cerebrospinal fluid, synovial fluid | Antibodies against an infectious agent 802 – Medical Microbiology |
Immunoblotting | Commercial procedure | |
| 23 | Cerebrospinal fluid, synovial fluid, tissue biopsies, urine, plasma, anticoagulated blood | Detection of nucleic acids of infectious agents 802 – Medical Microbiology |
Real-time PCR | Commercial procedure | |
| 24 | Serum, plasma | Antibodies against an infectious agent 802 – Medical Microbiology |
Indirect immunofluorescence | Commercial procedure | |
| National Reference Laboratory for Viral Hepatitis (NRL/VH) | |||||
| 25 | Body fluids | Antigens of infectious agents 802 – Medical Microbiology |
Immunoassay with photometric detection | Commercial procedure | |
| 26 | Body fluids | Antibodies against an infectious agent 802 – Medical Microbiology |
Immunoassay with photometric detection | Commercial procedure | |
| 27 | Body fluids | Detection of nucleic acids of infectious agents 802 – Medical Microbiology |
Real-time PCR | Commercial procedure | |
| 28 | Body fluids | Antigens of infectious agents 802 – Medical Microbiology |
Immunoassay with luminometric detection (automated) | Commercial procedure | |
| 29 | Body fluids | Antibodies against an infectious agent 802 – Medical Microbiology |
Immunoassay with luminometric detection (automated) | Commercial procedure | |
| 30 | Body fluids | Antibodies against an infectious agent 802 – Medical Microbiology |
Immunoblotting | Commercial procedure | |
| National Reference Laboratory for Meningococcal Infections (NRL/MEN) | |||||
| 31 | Bacterial culture | Identification of bacteria 802 – Medical Microbiology |
Phenotyping | Commercial procedure; published procedure | |
| 32 | Bacterial culture | Antigens of infectious agents 802 – Medical Microbiology |
Agglutination | Commercial procedure; published procedure | |
| 33 | Bacterial culture | Bacterial genotype 802 – Medical Microbiology |
Direct sequencing (Sanger) | Published procedure | |
| 34 | Clinical material | Detection of nucleic acids of infectious agents 802 – Medical Microbiology |
Real-time PCR | Published procedure | |
| National Reference Laboratory for Haemophilus Infections (NRL/HEM) | |||||
| 35 | Bacterial culture | Identification of bacteria 802 – Medical Microbiology |
Phenotyping | SOP-NRL/HEM-04, 1. 8. 2024 | |
| 36 | Bacterial culture | Antigens of infectious agents 802 – Medical Microbiology |
Agglutination | SOP-NRL/HEM-08, 1. 8. 2024 | |
| National Reference Laboratory for Streptococcal Infections (NRL/STR) | |||||
| 37 | Bacterial culture | Identification of bacteria 802 – Medical Microbiology |
Phenotyping | Commercial procedure; published procedure | |
| 38 | Bacterial culture | Identification of bacteria 802 – Medical Microbiology |
Agglutination | Commercial procedure | |
| 39 | Bacterial culture | Type antigens of Streptococcus pneumoniae 802 – Medical Microbiology |
Quellung reaction | Commercial procedure; published procedure | |
| 40 | Clinical material | Genotype of Streptococcus pyogenes 802 – Medical Microbiology |
Direct sequencing (Sanger) | Published procedure | |
| National Reference Laboratory for Influenza and Non-Influenza Respiratory Viral Diseases (NRL/CH a NCH) | |||||
| 41 | Clinical material | Detection of nucleic acids of infectious agents 802 – Medical Microbiology |
Real-time PCR | Commercial procedure; published procedure | |
| 42 | Clinical material | Detection of nucleic acids of infectious agents 802 – Medical Microbiology |
Nested PCR | Commercial procedure | |
| National Reference Laboratory for Syphilis Diagnostics (NRL/SYF) | |||||
| 43 | Clinical material | Anti-cardiolipin antibodies 802 – Medical Microbiology |
Agglutination | Commercial procedure | |
| 44 | Clinical material | Anti-treponemal antibodies 802 – Medical Microbiology |
Indirect passive agglutination | Commercial procedure | |
| 45 | Clinical material | Anti-treponemal antibodies 802 – Medical Microbiology |
Immunoassay with photometric detection | Commercial procedure | |
| 46 | Clinical material | Anti-treponemal antibodies 802 – Medical Microbiology |
Indirect immunofluorescence | Commercial procedure | |
| 47 | Clinical material | Anti-treponemal antibodies 802 – Medical Microbiology |
Immunoblotting | Commercial procedure | |
| 48 | Clinical material | Detection of nucleic acids of infectious agents 802 – Medical Microbiology |
Real-time PCR | Commercial procedure | |
| National Reference Laboratory for Detection of Infectious Agents by Electron Microscopy (NRL/ELM) | |||||
| 49 | Clinical material | Direct detection of viruses 802 – Medical Microbiology |
Transmission electron microscopy | Published procedure | |
| National Reference Laboratory for Enteroviruses (NRL/ENT) | |||||
| 50 | Clinical material | Detection of viruses 802 – Medical Microbiology |
Cultivation on tissue cultures | Published procedure | |
| 51 | Body fluids | Antibodies against an infectious agent 802 – Medical Microbiology |
Virus neutralisation test | Published procedure | |
| 52 | Body fluids | Antibodies against an infectious agent 802 – Medical Microbiology |
Immunoassay with photometric detection | Commercial procedure | |
| National Reference Laboratory for Herpesviruses (NRL/HV) | |||||
| 53 | Serum, plasma, cerebrospinal fluid | Antibodies against an infectious agent 802 – Medical Microbiology |
Immunoassay with photometric detection | SOP-NRL/HV-01, 1. 8. 2024 | |
| 54 | Serum, plasma | Antibodies against an infectious agent 802 – Medical Microbiology |
Indirect immunofluorescence | SOP-NRL/HV-03, 1. 8. 2024 | |
| 55 | Blood, cerebrospinal fluid, lesion swab, urine, bronchoalveolar lavage aspirate, conjunctival swab, leukocytes, autopsy material, infected tissue cultures, ocular fluid, punctates, bone marrow, amniotic fluid | Detection of nucleic acids of infectious agents 802 – Medical Microbiology |
Real-time PCR | SOP-NRL/HV- 07, 1. 8. 2024; CFX96 Touch; RotorGene QMDX5 plex | |
| 56 | Swabs, vesicular fluid, urine, bronchoalveolar lavage | Detection of herpesviruses 802 – Medical Microbiology |
Cultivation on tissue cultures | SOP-NRL/HV-04, 1. 8. 2024 | |
| National Reference Laboratory for Rubella, Measles, Mumps and Parvovirus B19 (NRL/ZSP) | |||||
| 57 | Clinical material | Antibodies against infectious agents 802 – Medical Microbiology |
Immunoassay with photometric detection | Commercial procedure | |
| 58 | Clinical material | Detection of nucleic acids of infectious agents 802 – Medical Microbiology |
Real-time PCR | Commercial procedure | |
| National Reference Laboratory for Chlamydiae (NRL/CHLA) | |||||
| 59 | Clinical material | Antibodies against an infectious agent 802 – Medical Microbiology |
Immunoassay with photometric detection | Commercial procedure | |
| 60 | Clinical material | Antibodies against an infectious agent 802 – Medical Microbiology |
Indirect immunofluorescence | Commercial procedure | |
| 61 | Clinical material | Antibodies against an infectious agent 802 – Medical Microbiology |
Immunoblotting | Commercial procedure | |
| 62 | Clinical material | Antibodies against an infectious agent 802 – Medical Microbiology |
Immunoassay with luminometric detection | Commercial procedure | |
| National Reference Laboratory for Leptospires (NRL/LEPT) | |||||
| 63 | Serum, plasma | Antibodies against leptospires 802 – Medical Microbiology |
Microscopic agglutination test | Published procedure | |
| National Reference Laboratory for Toxoplasmosis (NRL/TOX) | |||||
| 64 | Serum, plasma | Antibodies against an infectious agent 802 – Medical Microbiology |
Complement fixation reaction | Commercial procedure | |
| 65 | Serum, plasma | Antibodies against an infectious agent 802 – Medical Microbiology |
Immunoassay with photometric detection | Commercial procedure | |
| 66 | Serum, plasma | Antibody avidity 802 – Medical Microbiology |
Immunoassay with photometric detection | Commercial procedure | |
| 67 | Serum, plasma | Antibodies against an infectious agent 802 – Medical Microbiology |
Immunoblotting | Commercial procedure | |
| National Reference Laboratory for Staphylococci (NRL/ST) | |||||
| 68 | Bacterial culture | Identification of bacteria 802 – Medical Microbiology |
Phenotyping | SOP-NRL/ST-04, 15. 5. 2019 | |
| 69 | Bacterial culture | Detection of toxins 802 – Medical Microbiology |
Reverse passive latex agglutination | SOP-NRL/ST-05, 1. 3. 2021 | |
| 70 | Bacterial culture | Genotyping of bacteria 802 – Medical Microbiology |
End-point PCR with subsequent detection by gel electrophoresis | In-house procedure | |
| National Reference Laboratory for Salmonellae (NRL/SA) | |||||
| 71 | Bacterial culture | Identification of bacteria 802 – Medical Microbiology |
Agglutination | SOP-NRL/SA-04, 1. 2. 2024 | |
| 72 | Bacterial culture | Identification of bacteria 802 – Medical Microbiology |
Phenotyping | SOP-NRL/SA-05, 1. 2. 2024 | |
| National Reference Laboratory for E. coli and Shigellae (NRL/ECS) | |||||
| 73 | Bacterial culture | Identification of bacteria 802 – Medical Microbiology |
Phenotyping | Published procedure | |
| 74 | Bacterial culture | Identification of bacteria 802 – Medical Microbiology |
Agglutination | Commercial procedure | |
| 75 | Clinical material | Bacterial genotype 802 – Medical Microbiology |
End-point PCR with gel electrophoretic detection | Published procedure; in-house procedure | |
| National Reference Laboratory for Pertussis and Diphtheria (NRL/DIPE) | |||||
| 76 | Bacterial culture | Identification of bacteria 802 – Medical Microbiology |
Agglutination | Commercial procedure | |
| 77 | Body fluids | Antibodies against an infectious agent 802 – Medical Microbiology |
Immunoassay with photometric detection | Commercial procedure | |
| 78 | Bacterial culture | Identification of bacteria 802 – Medical Microbiology |
Phenotyping | Commercial procedure | |
| 79 | Clinical material | Detection of nucleic acids of infectious agents 802 – Medical Microbiology |
Real-time PCR | Commercial procedure | |
Reference standards (19)
CIE10.NRL/HIV/AIDS
SOP-NRL/HEM-04
SOP-NRL/HEM-08
SOP-NRL/HIV/AIDS-03
SOP-NRL/HIV/AIDS-05
SOP-NRL/HIV/AIDS-06
SOP-NRL/HIV/AIDS-07
SOP-NRL/HIV/AIDS-08
SOP-NRL/HIV/AIDS-15
SOP-NRL/HIV/AIDS-19
SOP-NRL/HIV/AIDS-21
SOP-NRL/HV- 07
SOP-NRL/HV-01
SOP-NRL/HV-03
SOP-NRL/HV-04
SOP-NRL/SA-04
SOP-NRL/SA-05
SOP-NRL/ST-04
SOP-NRL/ST-05