🇨🇿 Ústav hematologie a krevní transfuze PL
Other
Ústav hematologie a krevní transfuze is a laboratory accredited by ČIA under number 1345 (Praha 2, Czech Republic). Its accreditation scope covers 11 test methods in the following areas: Other. Test objects include: Biological material containing human DNA of the cell nuclei, Biological material containing human RNA. Most frequently used techniques: by PCR and gel electrophoresis, by Real-time PCR method, direct sequencing by Sanger method.
At a glance
- Accreditation number: ČIA 1345
- Methods in scope: 11
- Types of test objects: 2
- Techniques used: 6
- City: Praha 2, Czech Republic
- Valid until: 11-12-2029
- Accreditation number
- 1345
- Accreditation body
- ČIA
- Address
- U Nemocnice 2094/1, 128 00 Praha 2
- City
- Praha 2, Czech Republic
- Division
- Krajowe Laboratorium Referencyjne Diagnostyki DNA
- Valid until
- 11-12-2029
What this laboratory tests (2)
- Biological material containing human DNA of the cell nuclei — 7 methods
- Biological material containing human RNA — 4 methods
Analytical techniques used (6)
- by PCR and gel electrophoresis — 3 methods
- by Real-time PCR method — 3 methods
- direct sequencing by Sanger method — 1 method
- multiplex RT PCR — 1 method
- NGS-MPS — 1 method
- Real-time RT PCR — 1 method
Laboratory sections (3)
- Department of Cell Chimerism — 4 methods
- Department of Molecular Genetics — 4 methods
- Department of HLA — 3 methods
Laboratory locations
- Oddział Genetyki Molekularnej — U Nemocnice 2094/1
- Oddział HLA — Kateřinská 521/19
- Oddział Chimeryzmu Komórkowego — Kateřinská 521/19
Certificate information
- Certificate date
- 11.12.2024
Accreditation scope — test methods (11)
| No. | Test object | Methodology / Parameters | Technique | Reference document | |
|---|---|---|---|---|---|
| Department of Molecular Genetics | |||||
| 1 | Biological material containing human RNA | Determination of BCR::ABL1 fusion gene rearrangements using multiplex RT PCR Regulated area: Department of Molecular Genetics |
multiplex RT PCR | NRL_03_SOP_14_02 | |
| 2 | Biological material containing human RNA | Determination of BCR::ABL1 transcript level using Real-time RT PCR Regulated area: Department of Molecular Genetics |
Real-time RT PCR | NRL_04_SOP_14_02 postup A | |
| 3 | Biological material containing human RNA | Determination of BCR::ABL1 kinase domain mutation using direct sequencing by Sanger method Regulated area: Department of Molecular Genetics |
direct sequencing by Sanger method | NRL_04_SOP_14_02 postup B | |
| 4 | Biological material containing human RNA | Determination of the conversion factor for expressing the results of BCR::ABL1 transcript level on international scale Regulated area: Department of Molecular Genetics |
NRL_04_SOP_14_02 postup C | ||
| Department of HLA | |||||
| 5 | Biological material containing human DNA of the cell nuclei | Determination of gene sequence variants related to haematological and immunological diseases and hematopoietic stem cell transplant program by PCR and gel electrophoresis Regulated area: Department of HLA |
by PCR and gel electrophoresis | NRL_05_SOP_14_02 postup A | |
| 6 | Biological material containing human DNA of the cell nuclei | Determination of gene sequence variants related to haematological and immunological diseases and hematopoietic stem cell transplant program by Real-time PCR method Regulated area: Department of HLA |
by Real-time PCR method | NRL_05_SOP_14_02 postup C | |
| 7 | Biological material containing human DNA of the cell nuclei | Determination of gene sequence variants related to haematological and immunological diseases and hematopoietic stem cell transplant program by NGS-MPS Regulated area: Department of HLA |
NGS-MPS | NRL_05_SOP_14_02 postup D | |
| Department of Cell Chimerism | |||||
| 8 | Biological material containing human DNA of the cell nuclei | Genotype assessment of sequence polymorphisms by PCR and gel electrophoresis method Regulated area: Department of Cell Chimerism |
by PCR and gel electrophoresis | NRL_01_SOP_14_02 postup A | |
| 9 | Biological material containing human DNA of the cell nuclei | Assessment of quantitative representation of genotypes in a sample by analysis of sequence polymorphisms by PCR and gel electrophoresis method Regulated area: Department of Cell Chimerism |
by PCR and gel electrophoresis | NRL_01_SOP_14_02 postup B | |
| 10 | Biological material containing human DNA of the cell nuclei | Genotype assessment of sequence polymorphisms by Real-time PCR method Regulated area: Department of Cell Chimerism |
by Real-time PCR method | NRL_07_SOP_14_02 postup A | |
| 11 | Biological material containing human DNA of the cell nuclei | Assessment of quantitative representation of genotypes in a sample by analysis of sequence polymorphisms by Real-time PCR method Regulated area: Department of Cell Chimerism |
by Real-time PCR method | NRL_07_SOP_14_02 postup B | |
Reference standards (11)
NRL_01_SOP_14_02 postup A
NRL_01_SOP_14_02 postup B
NRL_03_SOP_14_02
NRL_04_SOP_14_02 postup A
NRL_04_SOP_14_02 postup B
NRL_04_SOP_14_02 postup C
NRL_05_SOP_14_02 postup A
NRL_05_SOP_14_02 postup C
NRL_05_SOP_14_02 postup D
NRL_07_SOP_14_02 postup A
NRL_07_SOP_14_02 postup B